CALLOT Virginie

Research Director (PhD)

at CNRS

Head of Spinal Cord MR Imaging research group

Coordinator of the IRL/LIA iLab-Spine

Research Director (PhD)

Download my Resume (EN)

Detailed Activities

The general objective of the Spinal Cord MR Imaging research group is to improve the non invasive characterization of the spinal cord impairments and to help in the description of the spinal cord pathophysiological mechanisms using quantitative and multi-parametric Magnetic Resonance Imaging.
The global project relies on methodological developments (MR techniques leading to structural and functional information, post-processing tools such as probabilistic MR atlas) and their applications to dedicated spinal cord pathologies. The project is developed transversally, on mouse models using the 11.75T MR system, and on humans using clinical research (1.5T, 3T) and ultra-high field (7T) MR systems.
Current pathologies under investigation include Multiple Sclerosis (MS) and Amyotrophic Lateral Sclerosis (ALS) in collaboration with the Neurology Department of La Timone Hospital (APHM, Pr. B. Audoin, Pr. J. Pelletier, Pr. S. Attarian, Dr. A. Verschueren), Cervicarthrosic Myelopathy (CSM) in collaboration with the Neurosurgery Department of the North Hospital (APHM, Pr. PH. Roche, Dr. A. Melot), NeuroMyelitis Optica Spectrum Disorders (NMOSD) in collaboration with the CHU Lyon (Pr. R. Marignier), as well as normal Ageing and sport-related microtraumatism such as encountered in Rugby.
One part of the project additionally combines MR and biomechanical computational modeling in order to provide an extensive and multi-physic description of traumatic and degenerative spinal cord pathologies (International Research Laboratory (IRL/LIA) “ iLab-Spine ”, France/Canada).

Keywords

  • Image Processing
  • Microstructure/architecture
  • New MR Biomarkers
  • Perfusion Methods
  • Quantitative MRI
  • Spinal Cord
  • Ultra-high field MRI
  • MR Templates
  • Morphometry
  • Diffusion
  • Probabilistic atlas
  • Neurodegenerative pathologies

General Information

. Co-director of the iLab-Spine (International Research Laboratory (IRL/LIA) on Spine Imaging and Biomechanics, France/Canada)

. Member of the CRMBM Unit Council

. Scientific Council member of the IRME (Institut pour la Recherche sur la Moelle Epinière)

. Vice Chair of the Club de Recherche sur la Moelle Epinière et ses Pathologies (CMEP)

. Board member of the SFRMBM (Société Française de Résonance Magnétique Biologique et Médicale) from 2012-2019

Research Projects

Publications :

Viallon, M, Y Berthezène, V Callot, M Bourgeois, H Humblot, A Briguet, and Y Crémillieux. “Dynamic Imaging of Hyperpolarized (3)He Distribution in Rat Lungs Using Interleaved-Spiral Scans.” NMR in Biomedicine 13, no. 4 (June 2000): 207–13.
Viallon, M, Y Berthezène, M Décorps, M Wiart, V Callot, M Bourgeois, H Humblot, A Briguet, and Y Crémillieux. “Laser-Polarized (3)He as a Probe for Dynamic Regional Measurements of Lung Perfusion and Ventilation Using Magnetic Resonance Imaging.” Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine 44, no. 1 (July 2000): 1–4.
Callot, V, E Canet, J Brochot, Y Berthezène, M Viallon, H Humblot, A Briguet, H Tournier, and Y Crémillieux. “Vascular and Perfusion Imaging Using Encapsulated Laser-Polarized Helium.” Magma (New York, N.Y.) 12, no. 1 (March 2001): 16–22.
Callot, V, E Canet, J Brochot, M Viallon, H Humblot, A Briguet, H Tournier, and Y Crémillieux. “MR Perfusion Imaging Using Encapsulated Laser-Polarized 3He.” Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine 46, no. 3 (September 2001): 535–40.
Callot, V, E Canet, J Brochot, H Humblot, A Briguet, H Tournier, and Y Crémillieux. “Hyperpolarized Helium3 Encapsulated in Microbubbles: A New Class of Blood Pool MRI Contrast Agent.” Academic Radiology 9 Suppl 2 (August 2002): S501-503.
Peces-Barba, G, J Ruiz-Cabello, Y Cremillieux, I Rodríguez, D Dupuich, V Callot, M Ortega, M L Rubio Arbo, M Cortijo, and N Gonzalez-Mangado. “Helium-3 MRI Diffusion Coefficient: Correlation to Morphometry in a Model of Mild Emphysema.” The European Respiratory Journal 22, no. 1 (July 2003): 14–19.
Callot, V, E Bennett, U K M Decking, R S Balaban, and H Wen. “In Vivo Study of Microcirculation in Canine Myocardium Using the IVIM Method.” Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine 50, no. 3 (September 2003): 531–40. https://doi.org/10.1002/mrm.10568.
Callot, V, D Galanaud, D Figarella-Branger, Y Lefur, P Metellus, F Nicoli, and P Cozzone. “Correlations between MR and Endothelial Hyperplasia in Low-Grade Gliomas.” Journal of Magnetic Resonance Imaging: JMRI 26, no. 1 (July 2007): 52–60. https://doi.org/10.1002/jmri.20995.
Callot, Virginie, Guillaume Duhamel, and Patrick J Cozzone. “In Vivo Mouse Spinal Cord Imaging Using Echo-Planar Imaging at 11.75 T.” Magma (New York, N.Y.) 20, no. 4 (October 2007): 169–73. https://doi.org/10.1007/s10334-007-0079-x.
Callot, V., G. Duhamel, and P. J. Cozzone. “Rapid and High-Resolved Diffusion Tensor Imaging of Mouse Lower Brain and Cervical Spinal Cord,” 2008. http://cds.ismrm.org/ismrm-2008/files/03395.pdf.
Callot, V., G. Duhamel, F. Kober, and P. J. Cozzone. “A Diffusion and Perfusion EPI-Based MR Protocol for the Characterization of Rodent Spinal Cord Diseases.,” 2008. http://cds.ismrm.org/ismrm-2008/files/03398.pdf.
Duhamel, G., V. Callot, F. Kober, and P. J. Cozzone. “Mouse Lumbar Spinal Cord Blood Flow (SCBF) Measurements by Arterial Spin Labeling.” In Proc. Intl. Soc. Mag. Reson. Med, 16:3397, 2008. http://cds.ismrm.org/ismrm-2008/files/03397.pdf.
Duhamel, Guillaume, Virginie Callot, Patrick J. Cozzone, and Frank Kober. “Spinal Cord Blood Flow Measurement by Arterial Spin Labeling.” Magnetic Resonance in Medicine 59, no. 4 (April 2008): 846–54. https://doi.org/10.1002/mrm.21567.
Callot, Virginie, Damien Galanaud, Yann Le Fur, Sylviane Confort-Gouny, Jean-Philippe Ranjeva, and Patrick J Cozzone. “(1)H MR Spectroscopy of Human Brain Tumours: A Practical Approach.” European Journal of Radiology 67, no. 2 (August 2008): 268–74. https://doi.org/10.1016/j.ejrad.2008.02.036.
Callot, Virginie, Guillaume Duhamel, Patrick J. Cozzone, and Frank Kober. “Short-Scan-Time Multi-Slice Diffusion MRI of the Mouse Cervical Spinal Cord Using Echo Planar Imaging.” NMR in Biomedicine 21, no. 8 (October 2008): 868–77. https://doi.org/10.1002/nbm.1274.
Callot, V., Y. Le Fur, G. Duhamel, P. J. Cozzone, and F. Kober. “In Vivo 1H-MR Spectroscopy of the Mouse Cervical Spinal Cord.,” 2009. http://cds.ismrm.org/protected/09MProceedings/files/01293.pdf.
Duhamel, Guillaume, Virginie Callot, Patrick Decherchi, Yann Le Fur, Tanguy Marqueste, Patrick J. Cozzone, and Frank Kober. “Mouse Lumbar and Cervical Spinal Cord Blood Flow Measurements by Arterial Spin Labeling: Sensitivity Optimization and First Application.” Magnetic Resonance in Medicine 62, no. 2 (August 2009): 430–39. https://doi.org/10.1002/mrm.22015.
Callot, Virginie, Guillaume Duhamel, Yann Le Fur, Patrick Decherchi, Tanguy Marqueste, Frank Kober, and Patrick J Cozzone. “Echo Planar Diffusion Tensor Imaging of the Mouse Spinal Cord at Thoracic and Lumbar Levels: A Feasibility Study.” Magnetic Resonance in Medicine 63, no. 4 (April 2010): 1125–34. https://doi.org/10.1002/mrm.22301.
Callot, Virginie, Guillaume Duhamel, and Frank Kober. “Spinal Cord - MR of Rodent Models.” In Methods in Molecular Biology (Clifton, N.J.), 771:355–83, 2011.
Kober, Frank, Guillaume Duhamel, and Virginie Callot. “Cerebral Perfusion MRI in Mice.” In Methods in Molecular Biology (Clifton, N.J.), 771:117–38, 2011. https://doi.org/10.1007/978-1-61779-219-9_6.
Fellah, S, N Girard, O Chinot, P J Cozzone, and V Callot. “Early Evaluation of Tumoral Response to Antiangiogenic Therapy by Arterial Spin Labeling Perfusion Magnetic Resonance Imaging and Susceptibility Weighted Imaging in a Patient with Recurrent Glioblastoma Receiving Bevacizumab.” Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology 29, no. 11 (April 10, 2011): e308-311. https://doi.org/10.1200/JCO.2010.32.6082.
Callot, V, D Galanaud, and P Cozzone. “Differentiation between Gliomatosis Cerebri and Low-Grade Glioma: Proton Magnetic Resonance Spectroscopy.” In Tumors of the Central Nervous System, 153–59. Elsevier, 2012. http://crmbm.univ-amu.fr/CALLOT-Virginie,156.
Fellah, Slim, Virginie Callot, Patrick Viout, Sylviane Confort-Gouny, Didier Scavarda, Philippe Dory-Lautrec, Dominique Figarella-Branger, Patrick J Cozzone, and Nadine Girard. “Epileptogenic Brain Lesions in Children: The Added-Value of Combined Diffusion Imaging and Proton MR Spectroscopy to the Presurgical Differential Diagnosis.” Child’s Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery 28, no. 2 (February 2012): 273–82. https://doi.org/10.1007/s00381-011-1604-9.
Duhamel, Guillaume, Virginie Callot, Mohamed Tachrount, David C Alsop, and Patrick J Cozzone. “Pseudo-Continuous Arterial Spin Labeling at Very High Magnetic Field (11.75 T) for High-Resolution Mouse Brain Perfusion Imaging.” Magnetic Resonance in Medicine 67, no. 5 (May 2012): 1225–36. https://doi.org/10.1002/mrm.23096.
Tachrount, Mohamed, Guillaume Duhamel, Jérôme Laurin, Tanguy Marqueste, André Maues de Paula, Patrick Decherchi, Patrick J Cozzone, and Virginie Callot. “In Vivo Short TE Localized (1) H MR Spectroscopy of Mouse Cervical Spinal Cord at Very High Magnetic Field (11.75 T).” Magnetic Resonance in Medicine 69, no. 5 (May 2013): 1226–32. https://doi.org/10.1002/mrm.24360.
Fellah, S., D. Caudal, A. M. De Paula, P. Dory-Lautrec, D. Figarella-Branger, O. Chinot, P. Metellus, et al. “Multimodal MR Imaging (Diffusion, Perfusion, and Spectroscopy): Is It Possible to Distinguish Oligodendroglial Tumor Grade and 1p/19q Codeletion in the Pretherapeutic Diagnosis?” AJNR. American Journal of Neuroradiology 34, no. 7 (July 2013): 1326–33. https://doi.org/10.3174/ajnr.A3352.
Fradet, Léo, Pierre-Jean Arnoux, Jean-Philippe Ranjeva, Yvan Petit, and Virginie Callot. “Morphometrics of the Entire Human Spinal Cord and Spinal Canal Measured from in Vivo High-Resolution Anatomical Magnetic Resonance Imaging.” Spine 39, no. 4 (February 15, 2014): E262-269. https://doi.org/10.1097/BRS.0000000000000125.
Duhamel, Guillaume, Valentin Prevost, Olivier M. Girard, Virginie Callot, and Patrick J. Cozzone. “High-Resolution Mouse Kidney Perfusion Imaging by Pseudo-Continuous Arterial Spin Labeling at 11.75T.” Magnetic Resonance in Medicine 71, no. 3 (March 1, 2014): 1186–96. https://doi.org/10.1002/mrm.24740.
Taso, Manuel, Arnaud Le Troter, Michaël Sdika, Jean-Philippe Ranjeva, Maxime Guye, Monique Bernard, and Virginie Callot. “Construction of an in Vivo Human Spinal Cord Atlas Based on High-Resolution MR Images at Cervical and Thoracic Levels: Preliminary Results.” Magma (New York, N.Y.) 27, no. 3 (June 2014): 257–67. https://doi.org/10.1007/s10334-013-0403-6.
Fonov, V. S., A. Le Troter, M. Taso, B. De Leener, G. Lévêque, M. Benhamou, M. Sdika, et al. “Framework for Integrated MRI Average of the Spinal Cord White and Gray Matter: The MNI-Poly-AMU Template.” NeuroImage 102 Pt 2 (November 15, 2014): 817–27. https://doi.org/10.1016/j.neuroimage.2014.08.057.
Taso, Manuel, Arnaud Le Troter, Michaël Sdika, Julien Cohen-Adad, Pierre-Jean Arnoux, Maxime Guye, Jean-Philippe Ranjeva, and Virginie Callot. “A Reliable Spatially Normalized Template of the Human Spinal Cord - Applications to Automated White Matter/Gray Matter Segmentation and Tensor-Based Morphometry (TBM) Mapping of Gray Matter Alterations Occurring with Age.” NeuroImage 117 (August 15, 2015): 20–28. https://doi.org/10.1016/j.neuroimage.2015.05.034.
Taso, M., L. Fradet, V. Callot, and P. J. Arnoux. “Anteroposterior Compression of the Spinal Cord Leading to Cervical Myelopathy: A Finite Element Analysis.” Computer Methods in Biomechanics and Biomedical Engineering 18 Suppl 1 (October 2015): 2070–71. https://doi.org/10.1080/10255842.2015.1069625.
Prevost, Valentin H., Olivier M. Girard, Virginie Callot, Patrick J. Cozzone, and Guillaume Duhamel. “Fast Imaging Strategies for Mouse Kidney Perfusion Measurement with Pseudocontinuous Arterial Spin Labeling (PCASL) at Ultra High Magnetic Field (11.75 Tesla).” Journal of Magnetic Resonance Imaging 42, no. 4 (October 1, 2015): 999–1008. https://doi.org/10.1002/jmri.24874.
Lévy, S., M. Benhamou, C. Naaman, P. Rainville, V. Callot, and J. Cohen-Adad. “White Matter Atlas of the Human Spinal Cord with Estimation of Partial Volume Effect.” NeuroImage 119 (October 1, 2015): 262–71. https://doi.org/10.1016/j.neuroimage.2015.06.040.
Fradet, Léo, Pierre-Jean Arnoux, Virginie Callot, and Yvan Petit. “Geometrical Variations in White and Gray Matter Affect the Biomechanics of Spinal Cord Injuries More than the Arachnoid Space.” Advances in Mechanical Engineering 8, no. 8 (2016): 168781401666470. https://doi.org/10.1177/1687814016664703.
De Leener, Benjamin, Manuel Taso, Julien Cohen-Adad, and Virginie Callot. “Segmentation of the Human Spinal Cord.” Magma (New York, N.Y.) 29, no. 2 (April 2016): 125–53. https://doi.org/10.1007/s10334-015-0507-2.
Taso, Manuel, Olivier M. Girard, Guillaume Duhamel, Arnaud Le Troter, Thorsten Feiweier, Maxime Guye, Jean-Philippe Ranjeva, and Virginie Callot. “Tract-Specific and Age-Related Variations of the Spinal Cord Microstructure: A Multi-Parametric MRI Study Using Diffusion Tensor Imaging (DTI) and Inhomogeneous Magnetization Transfer (IhMT).” NMR in Biomedicine 29, no. 6 (June 2016): 817–32. https://doi.org/10.1002/nbm.3530.
Massire, Aurélien, Manuel Taso, Pierre Besson, Maxime Guye, Jean-Philippe Ranjeva, and Virginie Callot. “High-Resolution Multi-Parametric Quantitative Magnetic Resonance Imaging of the Human Cervical Spinal Cord at 7T.” NeuroImage 143 (August 26, 2016): 58–69. https://doi.org/10.1016/j.neuroimage.2016.08.055.
Girard, Olivier M., Virginie Callot, Valentin H. Prevost, Benjamin Robert, Manuel Taso, Guilherme Ribeiro, Gopal Varma, Novena Rangwala, David C. Alsop, and Guillaume Duhamel. “Magnetization Transfer from Inhomogeneously Broadened Lines (IhMT): Improved Imaging Strategy for Spinal Cord Applications.” Magnetic Resonance in Medicine 77 (January 2017): 581–91. https://doi.org/10.1002/mrm.26134.
De Leener, Benjamin, Simon Levy, Sara M. Dupont, Vladimir S. Fonov, Nikola Stikov, D. Louis Collins, Virginie Callot, and Julien Cohen-Adad. “SCT: Spinal Cord Toolbox, an Open-Source Software for Processing Spinal Cord MRI Data.” Neuroimage 145 (January 15, 2017): 24–43. https://doi.org/10.1016/j.neuroimage.2016.10.009.
Dupont, Sara M., Benjamin De Leener, Manuel Taso, Arnaud Le Troter, Sylvie Nadeau, Nikola Stikov, Virginie Callot, and Julien Cohen-Adad. “Fully-Integrated Framework for the Segmentation and Registration of the Spinal Cord White and Gray Matter.” NeuroImage 150 (April 15, 2017): 358–72. https://doi.org/10.1016/j.neuroimage.2016.09.026.
De Leener, Benjamin, Gabriel Mangeat, Sara Dupont, Allan R. Martin, Virginie Callot, Nikola Stikov, Michael G. Fehlings, and Julien Cohen-Adad. “Topologically Preserving Straightening of Spinal Cord MRI.” Journal of Magnetic Resonance Imaging: JMRI 46, no. 4 (October 2017): 1209–19. https://doi.org/10.1002/jmri.25622.
Rasoanandrianina, Henitsoa, Aude-Marie Grapperon, Manuel Taso, Olivier M. Girard, Guillaume Duhamel, Maxime Guye, Jean-Philippe Ranjeva, Shahram Attarian, Annie Verschueren, and Virginie Callot. “Region-Specific Impairment of the Cervical Spinal Cord (SC) in Amyotrophic Lateral Sclerosis: A Preliminary Study Using SC Templates and Quantitative MRI (Diffusion Tensor Imaging/Inhomogeneous Magnetization Transfer).” NMR in Biomedicine 30, no. 12 (December 2017). https://doi.org/10.1002/nbm.3801.
Fournely, Marion, Yvan Petit, Éric Wagnac, Jérôme Laurin, Virginie Callot, and Pierre-Jean Arnoux. “High-Speed Video Analysis Improves the Accuracy of Spinal Cord Compression Measurement in a Mouse Contusion Model.” Journal of Neuroscience Methods 293 (January 1, 2018): 1–5. https://doi.org/10.1016/j.jneumeth.2017.09.007.
De Leener, Benjamin, Vladimir S. Fonov, D. Louis Collins, Virginie Callot, Nikola Stikov, and Julien Cohen-Adad. “PAM50: Unbiased Multimodal Template of the Brainstem and Spinal Cord Aligned with the ICBM152 Space.” NeuroImage 165 (January 15, 2018): 170–79. https://doi.org/10.1016/j.neuroimage.2017.10.041.
Gros, Charley, Benjamin De Leener, Sara M. Dupont, Allan R. Martin, Michael G. Fehlings, Rohit Bakshi, Subhash Tummala, et al. “Automatic Spinal Cord Localization, Robust to MRI Contrasts Using Global Curve Optimization.” Medical Image Analysis 44 (February 2018): 215–27. https://doi.org/10.1016/j.media.2017.12.001.
Benacchio, S., O. Doutres, A. Le Troter, A. Varoquaux, E. Wagnac, V. Callot, and F. Sgard. “Estimation of the Ear Canal Displacement Field Due to In-Ear Device Insertion Using a Registration Method on a Human-like Artificial Ear.” Hearing Research 365 (June 2, 2018): 16–27. https://doi.org/10.1016/j.heares.2018.05.019.
Massire, Aurélien, Henitsoa Rasoanandrianina, Manuel Taso, Maxime Guye, Jean-Philippe Ranjeva, Thorsten Feiweier, and Virginie Callot. “Feasibility of Single-Shot Multi-Level Multi-Angle Diffusion Tensor Imaging of the Human Cervical Spinal Cord at 7T.” Magnetic Resonance in Medicine 80, no. 3 (September 2018): 947–57. https://doi.org/10.1002/mrm.27087.
Gros, Charley, Benjamin De Leener, Atef Badji, Josefina Maranzano, Dominique Eden, Sara M. Dupont, Jason Talbott, et al. “Automatic Segmentation of the Spinal Cord and Intramedullary Multiple Sclerosis Lesions with Convolutional Neural Networks.” NeuroImage 184 (01 2019): 901–15. https://doi.org/10.1016/j.neuroimage.2018.09.081.
Eden, Dominique, Charley Gros, Atef Badji, Sara M. Dupont, Benjamin De Leener, Josefina Maranzano, Ren Zhuoquiong, et al. “Spatial Distribution of Multiple Sclerosis Lesions in the Cervical Spinal Cord.” Brain: A Journal of Neurology 142, no. 3 (01 2019): 633–46. https://doi.org/10.1093/brain/awy352.